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30ML Sputum Cup tells what is the reason for the upside-down when experimenting with 90mm Petri Dish?

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  • Time of issue:2021-03-18 11:46
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(Summary description)Mainly to dry its boards and facilitate operations such as streaking and coating. Inverted after inoculation is to prevent dripping and destroy the morphology of the colony.

30ML Sputum Cup tells what is the reason for the upside-down when experimenting with 90mm Petri Dish?

(Summary description)Mainly to dry its boards and facilitate operations such as streaking and coating. Inverted after inoculation is to prevent dripping and destroy the morphology of the colony.

  • Categories:News
  • Author:
  • Origin:
  • Time of issue:2021-03-18 11:46
  • Views:
Information

Will it be upside down when experimenting with 90mm Petri Dish? Let’s take a look at the 30ML Sputum Cup editor!
Mainly to dry its boards and facilitate operations such as streaking and coating. Inverted after inoculation is to prevent dripping and destroy the morphology of the colony. In fact, this effect is simple, in other words, if it is not managed, it will be very long and ugly, and it is impossible to count the number of colonies.
When inverted 90mm Petri Dish is cultivated, because the air inside is not easy to flow, the infection of foreign bacteria can be reduced as much as possible, and the contamination of the culture medium and culture by bacteria can be avoided. Through experimental observation, it is found that if the 90mm Petri Dish is placed in the correct direction, it is easy to grow contaminated bacterial colonies around.
When the culture conditions require ventilation, inversion can reduce the air flow on the surface of the medium, slow down the evaporation of water in the medium, keep the water in a solid medium such as agar, and fully maintain the elasticity of the medium. Agar and other coagulants prolong the time of medium rupture. Therefore, reverse culture is conducive to the reproduction and survival of bacteria. If the 90mm Petri Dish is placed vertically, the medium will lose water and break within a few days. It can be seen that it is very important to prevent water loss during the cultivation process.
When opening the incubator to take out and place the 90mm Petri Dish, it is easy to knock off the accidentally placed lid. Although the inverted one can maintain its original shape when removed, it is not easy to separate the bottom of the plate from the lid, thus avoiding the environment Bacteria contamination.
After turning it upside down, since the medium still has a residual temperature and has just solidified, the water content is relatively high and the humidity is relatively high, and some water droplets fall on the lid of the pan. If placed, the condensed water droplets will flow into the medium, which will not only cause pollution, but also separate the medium from it, making it easy to slide. This is one of the reasons why the medium cannot adhere firmly.
The main purpose of 90mm Petri Dish culture is to separate single-cell colonies for easy observation and identification. Turning them upside down can prevent condensation dripping onto the medium and avoid bacterial contamination. It is also conducive to the growth, reproduction and counting of bacterial species. .
The above is the 30ML Sputum Cup editor introduced the reasons for its upside-down, I hope it can help you.

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